Detection of Fluoroquinolones Antibiotic Resistant in Pseudomonas Aeruginosa Isolates

Abstract

Background: The Gram-negative bacterium Pseudomonas aeruginosa causes widespread morbidity and mortality. It enters the body through any weakness in the basic defenses of the human host.

Materials and Methods: This study designed to assess the correlation between the resistant of fluoroquinolones antibiotics in P. aeruginosa clinical isolates bacteria resulting from the excessive use of broad-spectrum antibiotics in hospitals. In the present study, forty clinical isolates (burn seven (17.5%), wound seven (17.5%), ear two (5%), operation room twelve (30%), urine three (7.5%), and nine (22.5%) from an industrial dialysis center were identified as P. aeruginosa using bacteriological diagnostic techniques. Nalidixic acid, ciprofloxacin, norfloxacin, ofloxacin, lomefloxacin, levofloxacin, and trovafloxacin are the seven antibiotics of the fluoroquinolones family that were used in the Kirby Bauer test to determine the antibiotic susceptibility of each isolate.

Results: Of the forty clinical isolates, three were sensitive and ten resistants to all tested antibiotics, while the other twenty-seven were intermediate, resistant, and sensitive to two or more tested antibiotics.

In conclusion: our findings revealed that highly resistant Pseudomonas aeruginosa strains have emerged as a major cause of opportunistic infections in hospitals due to the overuse of broad-spectrum antibiotics in hospitals.