Evaluation the Role of MIRNA-21 AND MIRNA-205 in Patients with Breast Cancer

Abstract

Background: Breast cancer is one of the most serious health problems and challenges facing women, as it is one of the most widespread tumors among women worldwide, and understanding molecular aspect is essential for improving diagnosis and treatment.

Objective: The aim of this research to investigate the impact of microRNAs (miR-205, miR-21) in breast cancer patients . and Comparing gene expression results between the breast cancer patients group and the control group and explains  the influence of increased or decreased gene expression of these parameters on tumor progression.

Materials and Methods: A total of 80 individuals, 40 from the healthy group and 40 from the control group, all females aged 30 to 50 and older, were sampled. Two milliliters of blood were collected in an EDTA tube for RNA extraction and genomic study.

Results: Achieved results indicated  that the gene expression of miR-205  was decreased significantly (P<0.01) in patients group which was (0.508±0.17 fold) in comparison to control group which was (1±0 fold) with the area under the curve (AUC) was found to be 0.826. while Achieved results indicated  that the gene expression of miR-21 was increased significantly (P<0.05)  in patients group which was( 1.72±0.43 fold) in comparison to control group which was (1±0fold )with thr area under the curve (AUC) was found (0.478).

Conclusion: The gene expression of miRNA-205 is decreased significantly in patients with breast cancer compared to the control group, ROC for miRNA-205 showed a good prognostic indicator for breast cancer patients with good sensitivity. while the gene expression of miR-21 is significantly higher in patients group compared to the healthy group. ROC for miRNA-21 demonstrated a good prognostic indicator for breast cancer patients with weak sensitivity.